Tropical Plant Pathology (2011) 36, 28-34

Daniel D.C. Carvalho, Sueli C.M. Mello, Murillo Lobo Júnior and Mayara C. Silva (2011)
Controle de Fusarium oxysporum f.sp. phaseoli in vitro e em sementes, e promoção do crescimento inicial do feijoeiro comum por Trichoderma harzianum
[Control of Fusarium oxysporum f.sp phaseoli in vitro and on seeds and growth promotion of common bean in early stages by Trichoderma harzianum]
Tropical Plant Pathology 36 (1), 28-34
Abstract: The objective of this study was to evaluate the antagonistic capacity of six isolates of Trichoderma harzianum against Fusarium oxysporum f.sp. phaseoli on infected seeds and its effect on the early stages of growth in bean plants. The isolates were also evaluated in dual culture assays and under in vitro antibiosis at 25ºC. Healthy and infected seeds were treated with 2 mL 100 g^-1 seeds of the antagonist suspensions (2.5 x 10⁸ conidia mL^-1) for healthy evaluation using blotter method at 20 and 25°C. The percentages of pathogen incidence and normal seedlings were evaluated at seven and nine days, respectively. The isolates were applied at 5 x 10⁹ conidia 500 g^-1 of sterilized substrate in greenhouse. After 11 days, roots and shoots of the bean plants were measured (12 plants treatment^-1). Every isolate of T. harzianum presented in vitro antagonism against the pathogen. The strains CEN202, CEN234, CEN238 and CEN240 offered better control of F. oxysporum on seeds than the positive check, causing a reduction between 35 and 51% in pathogen incidence and 73-81% of normal seedling emergence. The total length of the plants treated with CEN239 (37.43 cm) was longer than the other treatments, with length varying between 27.84 and 33.95 cm.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
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Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
evaluation - screening - selection

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium oxysporum f.sp. phaseoli	Beans (Phaseolus)
Trichoderma harzianum (antagonist)	Fusarium oxysporum f.sp. phaseoli	Beans (Phaseolus)
	Fusarium oxysporum