Plant Protection Science (2003) 39, 151-153

S. Sirca, G. Urek and V. Meglic (2003)
Molecular and biochemical methods used for the identification of Globodera species in Slovenia
Plant Protection Science 39 (4), 151-153
Abstract: Globodera species identification is based on specific morphological characteristics which are included in the majority of keys used for Globodera identification. To confirm and make more precise the morphometrical method, other methods have been developed. At Agricultural Institute of Slovenia, molecular and biochemical methods are applied. With the use of PCR-RFLP method we try to distinguish between G. rostochiensis, G. pallida and G. tabacum. Patterns of nematode DNA digested with restriction endonucleases and subjected to agarose gel electrophoresis are analysed. Differences in DNA sequence result in the number and size of fragments produced (RFLPs). For the differentiation of all three nematodes we have introduced a method for protein electrophoresis. Samples are compared after being separated by polyacrilamide slab gel electrophoresis. Proteins are visualised after silver staining.
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Database assignments for author(s): Sasa Sirca, Gregor Urek

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Globodera rostochiensis		Slovenia
	Globodera pallida		Slovenia
	Globodera tabacum		Slovenia