Phytopathology (1996) 86, p. S57 (Tigelaar et al.)

H. Tigelaar, M.H. Stuiver, L. Molendijk, E. Troost-van Deventer, M.B. Sela-Buurlage, J. Storms, L. Plooster, F. Sijbolts, J. Custers, M. Apotheker-de Groot and L.S. Melchers (1996)
Broad spectrum fungal resistance in transgenic carrot plants
Phytopathology 86 (11 suppl.), S57-S57
Abstract: Research at MOGEN is aimed at developing strategies for the control of fungal diseases in plants. One promising strategy consists of identifying antifungal proteins, and constitutive expression of the corresponding genes in transgenic plants. cDNAs and genomic DNA fragments encoding PR-proteins from tobacco were constitutively expressed in carrot lines. All tested constructs were able to some extent to increase resistance. Two types of constructs were more efficient in provoking resistance. One of these was an AP24 (PR-5, osmotin)-expressing construct, the other a two-gene construct in which both class 1-chitinase and -glucanase genes were modified. Field trials in two subsequent years have shown a substantial level of resistance against major pathogens of carrots. These include Altemaria dauci, Alternaria radicina, Cercospora carotae and Erysiphe heraclei. Most individual lines which had resistance against one pathogen exhibited significant resistance against all tested pathogens.

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Alternaria dauci	Carrot (Daucus carota)
	Alternaria radicina	Carrot (Daucus carota)
	Cercospora carotae	Carrot (Daucus carota)
	Erysiphe heraclei	Carrot (Daucus carota)