Pesquisa Agropecuária Brasileira (1999) 34, 1539-1544

Sérgio Arce Gomez, Flávio Moscardi and Daniel Ricardo Sosa-Gómez (1999)
Suscetibilidade de Spodoptera frugiperda a isolados geográficos de um vírus de poliedrose nuclear
[Spodoptera frugiperda susceptibility to nuclear polyhedrosis virus geographical isolates]
Pesquisa Agropecuária Brasileira 34 (9), 1539-1544
Abstract: In order to verify the Spodoptera frugiperda (Smith,1797) second instar larvae susceptibility to seven nuclear polyhedrosis virus (NPV) geographical isolates, seven bioassays were carried out at Embrapa-Centro Nacional de Pesquisa de Soja, Insect Pathology Laboratory, Londrina, Paraná State, Brazil. Artificial diet containing 0 (control), 2x10³, 4x10³, 8x10³, 16x10³, 32x10³, and 64x10³ polyhedral inclusion bodies (PIB)/mL was prepared for each virus isolate; each dose was offered, in 50 mL plastic cups to the larvae under controlled conditions (temperature 26±2ºC; relative humidity: 60±10% and photophase: 14 hours). The statistical analysis (Probits) was made on the total of dead larvae (daily scored from the fifth up to 14^th day after the inoculations). It was determined on base of non-overlapping of the 95% fiducial limits of the mean lethal concentrations (LC₅₀) that the isolate from Sertaneja, PR, was the most virulent (5,631 PIB/mL). The ones from Guatemala (11,520 PIB/mL), Ponta Grossa, PR (14,184 PIB/mL), Argentina (15,891 PIB/mL) and Alabama, USA (17,558 PIB/mL) were similar, but the Guatemala's isolate was superior to those from Louisiana, USA (19,325 PIB/mL) and Sete Lagoas, MG (25,310 PIB/mL). There were no significant differences among isolates, regarding the mean lethal time (LT₅₀): 8.3 to 10 days.
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(original language: Portuguese)
Full text of article
Database assignments for author(s): Daniel R. Sosa-Gomez, Flavio Moscardi

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Spodoptera frugiperda
Alphabaculovirus spofrugiperdae (entomopathogen)	Spodoptera frugiperda		Argentina
Alphabaculovirus spofrugiperdae (entomopathogen)	Spodoptera frugiperda		Brazil (south)