Parasitology Research (2009) 105, 97-111

Mario Canales, Victoria Naranjo, Consuelo Almazán, Ricardo Molina, Suzana A. Tsuruta, Matias P.J. Szabó, Raúl Manzano-Roman, José M. Pérez de la Lastra, Katherine M. Kocan, María Isabel Jiménez, J. Lucientes, M. Villar and J. de la Fuente (2009)
Conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin
Parasitology Research 105 (1), 97-111
Abstract: The control of arthropod vectors of pathogens that affect human and animal health is important for the eradication of vector-borne diseases. The ortholog of the tick-protective antigen, subolesin, was identified in Aedes albopictus and found to have conserved epitopes in ticks and mosquitoes. RNA interference with the tick and mosquito double-stranded RNA in three tick species resulted in significant gene knockdown and decreased tick weight and/or survival. Feeding Anopheles atroparvus, Aedes caspius, and Culex pipiens female mosquitoes on an A. albopictus subolesin hyperimmune serum resulted in 11 ± 5% to 29 ± 6% survival inhibition when compared to controls fed on preimmune serum. Feeding sand flies, Phlebotomus perniciosus, on antimosquito subolesin ortholog protein antibodies inhibited female survival and the number of larvae and adults obtained after hatching by 28 ± 22% and 16 ± 3%, respectively, when compared to controls. Vaccination with tick and mosquito subolesin ortholog proteins significantly reduced Ixodes scapularis tick infestation and weight in a similar way. However, vaccination with the recombinant mosquito subolesin ortholog antigen did not protect against Amblyomma americanum and Rhipicephalus sanguineus tick infestations. Collectively, these preliminary results provided the first evidence that development of vaccines may be possible for control of multiple arthropod vectors using subolesin orthologs but suggested that multiple antigens may be required to produce an effective vaccine.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): José de la Fuente, Matias P.J. Szabó, Javier Lucientes, Katherine M. Kocan

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ixodes scapularis
	Rhipicephalus sanguineus
	Culex pipiens
	Aedes albopictus
	Amblyomma americanum
	Aedes caspius
	Phlebotomus perniciosus
	Anopheles atroparvus