New Zealand Journal of Zoology (1992) 19, 133-138

John W. Marshall and Allan M. Crawford (1992)
A DNA probe which distinguishes Globodera pallida Pa2/3 from G. rostochiensis Ro1 and other cyst-forming nematodes
New Zealand Journal of Zoology 19 (3-4), 133-138
Abstract: A small DNA fragment (approx. 350 base pairs) from the genome of the potato cyst nematode Globodera pallida Pa2/3 was cloned in a bacterial plasmid. When used as a probe in dot-blot DNA hybridisations against a range of nematodes, the cloned DNA bound to G. pallida Pa2/3 but not to Globodera rostochiensis Rol. The cereal cyst nematode Heterodera avenae, the clover cyst nematode Heterodera trifolii, the root knot nematodes Meloidogyne hapla and Meloidogyne incognita, and the beet cyst nematode Heterodera schachtii did not cross-hybridise. This probe can detect as few as six larvae of G. pallida.
(The abstract has been supplied by the author or ISPI and is excluded from the Creative Commons licence.)
(original language: English)
Link to article at publishers website
Database assignments for author(s): Allan M. Crawford

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Globodera pallida	Potato (Solanum tuberosum)	New Zealand
	Globodera rostochiensis	Potato (Solanum tuberosum)	New Zealand