New Zealand Journal of Zoology (1983) 10, 405-407

Allan M. Crawford, Malcolm Parslow and Catherine Sheehan (1983)
Changes in the karyotype of the cell line, DSIR-HA-1179, and a comparison with that of its parent insect, Heteronychus arator (F.) (Coleoptera: Scarabaeidae)
New Zealand Journal of Zoology 10 (4), 405-407
Abstract: Eighteen months after establishment of the primary cell culture, 36 metaphase nuclei of cell line DSIR-HA-1179, derived from the black beetle, Heteronychus arator (F.), had between 32 and 43 chromosomes; one cell contained 67 chromosomes. Structural abnormalities such as double minute chromosomes, ring and di- and tri-centric chromosomes were observed. Thirty-six months after establishment the chromosomes appeared to be similar, but the number of chromosomes per cell was much more variable (range, 12–73; 42 metaphase cells). The modal chromosome number of testicular tissues from H. arator was 2n = 20 — including an Xy pair — which is characteristic of the Scarabaeidae. The karyotype of DSIR-HA-1179 cells is unstable, with the number of chromosomes becoming more variable with continued propagation.
(The abstract has been supplied by the author or ISPI and is excluded from the Creative Commons licence.)
(original language: English)
Link to article at publishers website
Database assignments for author(s): Allan M. Crawford

Research topic(s) for pests/diseases/weeds:
rearing/culturing/mass production

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Heteronychus arator