Neotropical Entomology (2010) 39, 767-773
Diego H. Sauka, Regina E. Basurto-Ríos, Jorge E. Ibarra and Graciela B. Benintende (2010)
Characterization of an Argentine isolate of Bacillus thuringiensis similar to the HD-1 strain
Neotropical Entomology 39 (5), 767-773
Abstract: We report the characterization of an Argentine isolate of Bacillus thuringiensis (INTA TA24-6) similar to the HD-1 strain, which harbors a cryptic cry2Ab gene that apparently is transcribed but not translated into a protein. INTA TA24-6 showed a Rep-PCR pattern identical to the HD-1 strain, a plasmid pattern that resembled that of this strain and cry1 and cry2 genes as HD-1. Screening of cry1 and cry2 genes showed that INTA TA24-6 harbors only cry1Ac and cry2Ab genes. Furthermore, crystalline inclusions of INTA TA24-6 exhibit a bipyramidal shape, typical of Lepidoptera-active B. thuringiensis strains, containing a major protein of ca. 130 kDa toxic to Epinotia aporema Wals. (Lepidoptera: Tortricidae) larvae. Neither the flat-square to cuboidal crystal nor a ca. 65 kDa protein typical of strains expressing Cry2 proteins were detected in INTA TA24-6. In agreement with this information, parasporal crystals of INTA TA24-6 did not show toxicity to Aedes aegypti L. (Diptera:Culicidae) larvae. Gene transcription analyses suggested that the cry2A gene might be cryptic in INTA TA24-6 despite its transcription at different sporulation stages.
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Database assignments for author(s): Diego H. Sauka, Jorge E. Ibarra
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Epinotia aporema | Argentina | |||
| Bacillus thuringiensis Cry1A-toxin (entomopathogen) | Epinotia aporema | Argentina | ||
| Bacillus thuringiensis Cry2A-toxin (entomopathogen) | Epinotia aporema | Argentina |
