Memórias do Instituto Oswaldo Cruz (2013) 108, 110-122
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COI barcode versus morphological identification of Culex ( Culex ) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil
Memórias do Instituto Oswaldo Cruz 108 (suppl.1), 110-122
Abstract: Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex ( Culex ) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species ( Cx. acharistus , Cx. chidesteri , Cx. dolosus , Cx. lygrus and Cx. saltanensis ) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus . Cx. maxi , Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex ( Cux. ) species.
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Database assignments for author(s): Maria Anice Mureb Sallum
Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant. |
|---|---|---|---|---|
| Culex quinquefasciatus | ||||
| Culex pipiens | ||||
| Culex coronator | ||||
| Culex declarator | ||||
| Culex tatoi | ||||
| Culex mollis | ||||
| Culex dolosus | ||||
| Culex maxi | ||||
| Culex apicinus | ||||
| Culex interfor | ||||
| Culex brethesi | ||||
| Culex eduardoi | ||||
| Culex lygrus | ||||
| Culex saltanensis |
