Memórias do Instituto Oswaldo Cruz (2005) 100, 495-500

Cong Li and Richard C. Wilkerson (2005)
Identification of Anopheles (Nyssorhynchus) albitarsis complex species (Diptera: Culicidae) using rDNA internal transcribed spacer 2-based polymerase chain reaction primes
Memórias do Instituto Oswaldo Cruz 100 (5), 495-500
Abstract: Anopheles (Nyssorhynchus) marajoara is a proven primary vector of malaria parasites in Northeast Brazil, and An. deaneorum is a suspected vector in Western Brazil. Both are members of the morphologically similar Albitarsis Complex, which also includes An. albitarsis and an undescribed species, An. albitarsis "B". These four species were recognized and can be identified using random amplified polymorphic DNA (RAPD) markers, but various other methodologies also point to multiple species under the name An. albitarsis. We describe here a technique for identification of these species employing polymerase chain reaction (PCR) primers based on ribosomal DNA internal transcribed spacer 2 (rDNA ITS2) sequence. Since this method is based on known sequence it is simpler than the sometimes problematical RAPD-PCR. Primers were tested on samples previously identified using RAPD markers with complete correlation.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Richard C. Wilkerson

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles albitarsis		Argentina
	Anopheles albitarsis		Brazil (south)
	Anopheles albitarsis		Brazil (NE)
	Anopheles marajoara		Venezuela
	Anopheles marajoara		Brazil (NW)
	Anopheles marajoara		Brazil (NE)
	Anopheles deaneorum		Argentina
	Anopheles deaneorum		Brazil (NW)