Medical Entomology and Zoology (2008) 59, 33-46
Osamu Komagata, Shinji Kasai, Itsumi Obara, Naoki Motoyama, Ikuo Tanaka, Mutsuo Kobayashi and Takashi Tomita (2008)
Concomitant identification of subspecies and insecticide resistance-associated mutations in the mosquito Culex pipiens complex by primer extension-based genotyping
Medical Entomology and Zoology 59 (2), 33-46
Abstract: Management of insecticide resistance in the mosquito Culex pipiens complex is important because this complex is a major vector of lymphatic filariasis and West Nile fever, which occurs worldwide, and has been exposed to frequent chemical controls with a variety of insecticides. We applied the SNaPshot assay, a primer extension-based genotyping method, to the identification of both insecticide resistance-associated point mutations at insecticidal active sites and the mosquito subspecies distributed in Japan. Variations at Leu1014 and Va11016 positions in the sodium channel and Ser119 in Drosophila Ace-paralogous acetylcholiesterase (p-Ace) were individually tested for the detection of mutations conferring decreased sensitivity to nerve insecticidal targets. Moreover, sequence differentiations in Drosophila Ace-orthologous acetylcholinesterase (o-Ace) gene were concomitantly tested for the detection of subspecies of Cx. pipiens pallens, Cx. p. molestus and Cx. quinquefasciatus. The exactness of the SNaPshot assay was verified using standard laboratory strains and mutagenized synthesized DNA. The assay was then applied to laboratory-colonized mosquitoes that were captured from Kyushu and Honshu, Japan. Judgments for both Cx. p. m. and Cx. p. pallens by SNaPshot assay agreed with the respective autogenicities of the colonies tested, except for an erroneous individual. Mutation of Leu1014 to Phe and Ser in the sodium channel genes were specifically identified in the samples of Cx. p. m. and Cx. p. pallens, respectively. No mutation was detected for the other target sites.
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Database assignments for author(s): Takashi Tomita, Mutsuo Kobayashi, Shinji Kasai
Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Culex quinquefasciatus | ||||
| Culex pipiens | Japan | |||
| Culex pipiens | Japan |
