Journal of Virology (2019) 93 (9 - e01822-18)

Edwige Berthelot, Marie Ducousso, Jean-Luc Macia, Florent Bogaert, Volker Baecker, Gaël Thébaud, Romain Gallet, Michel Yvon, Stéphane Blanc, Mounia Khelifa and Martin Drucker (2019)
Turnip mosaic virus is a second example of a virus using transmission activation for plant-to-plant propagation by aphids
Journal of Virology 93 (9 - e01822-18)
Abstract: Cauliflower mosaic virus (CaMV; family Caulimoviridae) responds to the presence of aphid vectors on infected plants by forming specific transmission morphs. This phenomenon, coined transmission activation (TA), controls plant-to-plant propagation of CaMV. A fundamental question is whether other viruses rely on TA. Here, we demonstrate that transmission of the unrelated turnip mosaic virus (TuMV; family Potyviridae) is activated by the reactive oxygen species H₂O₂ and inhibited by the calcium channel blocker LaCl₃. H₂O₂-triggered TA manifested itself by the induction of intermolecular cysteine bonds between viral helper component protease (HC-Pro) molecules and by the formation of viral transmission complexes, composed of TuMV particles and HC-Pro that mediates vector binding. Consistently, LaCl₃ inhibited intermolecular HC-Pro cysteine bonds and HC-Pro interaction with viral particles. These results show that TuMV is a second virus using TA for transmission but using an entirely different mechanism than CaMV. We propose that TuMV TA requires reactive oxygen species (ROS) and calcium signaling and that it is operated by a redox switch.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Gaël Thébaud, Stéphane Blanc, Martin Drucker

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Turnip mosaic virus