Journal of Virology (1979) 31, 190-198

Martha Brown, Allan M. Crawford and Peter Faulkner (1979)
Genetic analysis of a baculovirus, Autographa californica nuclear polyhedrosis virus I. Isolation of temperature-sensitive mutants and assortment into complementation groups
Journal of Virology 31 (1), 190-198
Abstract: Temperature-sensitive (ts) mutants were isolated from the baculovirus Autographa californica (alfalfa looper) MNPV, grown in Spodoptera frugiperda (fall armyworm) cells in the presence of N-methyl-N′-nitro-N-nitrosoguanidine. Of 567 plaque isolates screened, 27 were temperature sensitive (ts), representing a mutation frequency of 4.8%. Ten ts mutants were studied in detail: six failed to yield nonoccluded virus at 33°C (NOV mutants), whereas the other four produced nonoccluded virus but were restricted in formation of polyhedra at 33°C (Poly mutants). One of the six NOV mutants failed to synthesize viral DNA. Reversion and leak frequencies were determined, and the mutants were assorted into complementation groups based on the yield of polyhedrin synthesis in cells coinfected with pairs of mutants at 33°C, as measured by radioimmunoassay. For NOV mutants, complementation indexes were also based on virus yield and were consistent with those based on polyhedrin synthesis. Nine mutants were assorted into five complementation groups. One mutant remained unclassified.
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(original language: English)
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Database assignments for author(s): Allan M. Crawford

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
environment/habitat manipulation
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Autographa californica nucleopolyhedrovirus (entomopathogen)