Journal of Plant Pathology (2006) 88, p. S39 (Comes et al.)

S. Comes, A. Fanigliulo, R. Pacella, G. Parrella, L. Scassillo and A. Crescenzi (2006)
Recovery and characterization of Tomato yellow leaf curl Sardinia virus and its vector in the Basilicata Region
Journal of Plant Pathology 88 (3, Special Issue), S39-S39
S.I.Pa.V XIII National Meeting - Foggia, 12-16 September 2006 - Poster
Abstract: Tomato yellow leaf curl disease (TYLCD) is one of the most damaging diseases of tomato worldwide. Several virus species belonging to the genus Begomovirus, family Geminiviridae, have been reported to cause TYLCD. Two TYLC virus species are known to cause TYLCD in Italy: Tomato yellow curl virus (TYLCV, formerly TYLCV-Israel) and Tomato yellow leaf curl Sardinia virus (TYLCSV, formerly TYLCV-Sardinia). During autumn 2005 a serious disease was observed in protected tomato crops in the Ionian coast of Basilicata (Southern Italy). Symptoms consisted in marginal leaf yellowing, leaf curling, stunting and flower abortion. The disease was detected in a group of greenhouses with an incidence of infection up to 100%. Since the symptoms were similar to those described for TYLCSV and TYLCV, ELISA and molecular detection assays for these viruses were used. A total of 50 tomato samples were tested for virus infection and for the presence of vector. A couple of synthetic oligonucleotides allowing the amplification of the whole coat protein gene was used for PCR. RFLP analysis of the PCR product showed the presence of only TYLCSV. A PCR-amplified 1008 bp fragment from one of the symptomatic plants was cloned and sequenced, and was found to share a 99% similarity with the Italian TYLCSV isolate (accession number Z28390). The molecular characterization of the COI gene (citocrome oxidase I) from the populations of Bemisia tabaci associated with the TYLCD showd that B biotype was associated with TYLCSV epidemics.
Database assignments for author(s): Aniello Crescenzi, Giuseppe Parrella

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bemisia tabaci biotype MEAM1	Tomato (Solanum lycopersicum)	Italy
	Tomato yellow leaf curl Sardinia virus	Tomato (Solanum lycopersicum)	Italy