Journal of Plant Pathology (2006) 88, p. S33 (Bianchi et al.)
L. Bianchi, A.R. Babini, C. Ratti, L. Giunchedi and C. Rubies Autonell (2006)
Survey of fruit tree viruses on certified propagation materials in northern Italy
Journal of Plant Pathology 88 (3, Special Issue), S33-S33
S.I.Pa.V XIII National Meeting - Foggia, 12-16 September 2006 - Poster
Abstract: In Italy fruit tree nursery production is a very important activity regulated by a phytosanitary certification law (D.M 24/07/03). In 2005, we studied the incidence of viruses in candidate, nuclear and propagation mother plants, included in the virus-free certification programs of Emilia-Romagna and Piedmont regions. Samples (311) were first analyzed by indexing and serological assays. All accessions were virus-free when tested by DAS-ELISA, although uncertain results were obtained for 90 samples, while with indexing viral symptoms were observed in 8 samples. Molecular analysis (RT-PCR) was then made to investigate virus presence in DAS-ELISA doubtful samples and in symptomatic indicators. Eleven samples from candidate mother plants were infected, while one infected sample was from a propagation mother plant. In particular, 3 accessions (apple and plum) were infected by Apple chlorotic leaf spot virus (ACLSV), 7 (apple and pear) by Apple stem pitting virus (ASPV), 4 (apple and pear) by Apple stem grooving virus (ASGV), 3 (cherry) by Cherry green ring mottle virus (CGRMV), and 2 (apricot and plum) by Prunus necrotic ringspot virus (PNRSV). Additionally, one indicator was infected by ASGV. Mixed infection by ASPV, ACLSV and ASGV was only observed in one sample as was ACLSV/ASPV and ACLSV/PNRSV. Two samples had a mixed ASPV/ASGV infection. No Apricot latent virus (ApLV), Prune dwarf virus (PDV) or Plum poxvirus (PPV) infections were detected. This survey confirms the high sanitary quality (96%) of plant material produced in Northern Italy thanks to the regional phytosanitary service activity.
Database assignments for author(s): Concepcion Rubies Autonell
Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
quarantine treatments/regulations/aspects
