Journal of Plant Pathology (2005) 87, p. 302b (Parrella et al.)

G. Parrella, A. De Stradis, C. Vovlas and G.E. Agosteo (2005)
Outbreaks of Onion yellow dwarf virus in onion crops in Calabria
Journal of Plant Pathology 87 (4), 302-302
XII Meeting, Italian Society for Plant Pathology, Reggio Calabria, 29/9 - 1/10, 2005 (poster)
Abstract: In 2003-2004, chlorotic stripes, yellowing and curling of the leaves were observed in several onion (Allium cepa L., cv Cipolla rossa di Tropea) cultivations of Calabria (Lamezia Terme and Ricadi). Diseased plants ranged from 20 to 100%, depending on the field inspected. Electron microscope observations of leaf-dip preparation showed the presence of potyvirus-like particles about 780 nm in length. Attempts to infect mechanically Nicotiana spp. and Chenopodium spp. failed, but symptoms were reproduced only in onion plantlets inoculated when young. From a set of primer pairs directed against three common viruses infecting Allium spp., i.e. Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV) and Garlic common latent virus (GarCLV), expected amplicons were obtained only with the primer pair specific for OYDV was used. The 280 bp amplicon, corresponding to the C-terminus of the coat protein gene and part of 3' UTR of OYDV, were cloned and sequenced. Highest similarity was obtained with a strain of OYDV from Holland. Further confirmations of OYDV spread in onion cultivations of Calabria were obtained by dot blot hybridization of sap samples with a specific OYDV riboprobe. Moreover, virus particles were decorated only by IgG raised to OYDV coat protein. Since in some fields the disease affected the totality of the crop, infections by OYDV must be considered as a relevant problem for onion cultivation in Calabria, especially in those areas where the production of local varieties is of high economic relevance.
Database assignments for author(s): Giuseppe Parrella, Angelo De Stradis

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
population dynamics/ epidemiology

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Onion yellow dwarf virus	Onion/garlic/leek (Allium)	Italy
	Onion yellow dwarf virus	Onion/garlic/leek (Allium)	Netherlands