Journal of Plant Pathology (2004) 86, p. 325 (Marra et al.)
R. Marra, P. Ambrosino, V. Scala, C. Romano, F. Vinale, S. Ferraioli, M. Ruocco, V. Carbone, S.L. Woo, D. Turrà, F. Scala and M. Lorito (2004)
Proteomic study of three component interactions: plant, pathogens and antagonistic fungi
Journal of Plant Pathology 86 (4), 325-325
XI Meeting, Italian Society for Plant Pathology, Milan, 29/9 - 1/10, 2004 (poster)
Abstract: The molecular factors involved in the three-way interaction plant-pathogenic fungi - antagonistic/biocontrol fungi are still poorly understood. The aim of this work is to investigate the components involved in this interaction. 2-D maps of the protein extracts from the single components in various interactions between plants (potato, bean, tobacco or tomato), pathogens (Botrytis cinerea, Rhizoctonia solani or Pythium ultimum) and biocontrol fungi (Trichoderma atroviride P1 or Trichoderma harzianum T22) were obtained. The extracted proteins were separated by isoelectrofocusing followed by SDS-PAGE. During the in vitro interaction of T. harzianum strain T22 with tomato and the culture filtrate or cell walls of the pathogens, the spot number was higher than in the presence of pathogen biomass. In terms of Trichoderma differential proteins displayed on 2D gels, the most important changes were obtained in the presence of P. ultimum. During the in vivo interaction with tomato, the antagonist proteome changed much more in the presence of the soilborne fungi R. solani and P. ultimum than with the foliar fungus B. cinerea, both in terms of total, increased, or novel spots. In silico analysis of some of those spots revealed homology with intracellular enzymes (GTPases, hydrolases) and with stress-related proteins (heat shock proteins HSP70, bacteriocin, cloacin). In the plant proteome, pathogenesis-related proteins have been identified during the in vivo interaction of bean with R. solani and T. atroviride strain P1. This is in agreement with the demonstrated ability of these beneficial fungi to induce plant systemic disease resistance by activating expression of defence-related genes. Proteins extracted from T. atroviride strain P1 that were analysed by mass spectrometry, showed some interesting homologies with a fungal hydrophobin of Pleurotus ostreatus and an ABC transporter of Ralstonia metallidurans.
Database assignments for author(s): Sheridan L. Woo, Francesco Vinale, Felice Scala
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
general biology - morphology - evolution
