Journal of Plant Pathology (2003) 85, 313-314

G. Parrella, A. De Stradis and C. Vovlas (2003)
Tobacco mild green mosaic virus in Nicotiana glauca in south Italy
Journal of Plant Pathology 85 (4), 313-314
X Meeting, Italian Society for Plant Pathology (poster)
Abstract: Tobacco mild green mosaic virus (TMGMV; genus Tobamovirus) occurs probably in all tropical and subtropical regions where Nicotiana glauca (tobacco tree) is distribuited (North America, Australia, many European and African countries, Canary and Mediterranean islands). In Italy, TMGMV has been reported in pepper in 1970. During spring 2003 several plants of N. glauca growing in south Italy (Naples), were found to be infected by a tobamo-like virus based on preliminary electron-microscope observation of leaf samples. In immuno-diffusion agar tests sap extracts from symptomatic leaves of N. glauca formed a clear precipiting line with a serum to an American isolate of TMGMV (kindly provided by H. Elliot), while no reaction was observed with a serum to a common isolate of Tobacco mosaic virus (TMV). Molecular hybridization confirmed the absence of TMV in symptomatic N. glauca plants. The CP and the 3' UTR sequence of a TMGMV isolate (TMGMV-GNA) were determined from a single symptomatic N. glauca plant. The CP sequence confirmed that TMGMV-GNA is an isolate of TMGMV while 3' UTR sequence revealed the presence of an additional repeat sequence of 147 nt in the part of 3' UTR that is involved in the formation of six pseudoknots in the Large-type TMGMV isolates (three pseudoknots= Small-type TMGMV isolates). The host range of TMGMV-GNA was in agreement with that reported for TMGMV Large-type isolates. Dot-blot hybridization tests with a specific riboprobe for Large-type isolates showed that all symptomatic N. glauca plants harbor exclusively Large-type isolates of TMGMV.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Giuseppe Parrella, Angelo De Stradis

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tobacco mild green mosaic virus		Italy