Journal of Phytopathology - Phytopathologische Zeitschrift (1998) 146, 623-629

S. Bak, G.B. Poulsen and M. Albrechtsen (1998)
Two cases of non-host resistance against potyviruses operating at the single cell level
Journal of Phytopathology - Phytopathologische Zeitschrift 146 (11-12), 623-629
Abstract: Nonhost resistance of plants against viruses is a very common but poorly understood phenomenon. In this study Nicotiana tabacum L. and pea seed-borne mosaic potyvirus (PSbMV) were chosen as a model system for a closer examination of nonhost resistance. Nicotiana tahacum was confirmed to be a nonhost for PSbMV by mechanical inoculations followed by analysis by enzyme-linked immunosorbent assay and by immunofluorescence staining of mesophyll and epidermal protoplasts isolated from inoculated leaves. When tobacco protoplasts were inoculated with PSbMV virions or genomic RNA, only very few cells became infected. On the other hand, both PSbMV virions and genomic RNA could infect protoplasts from the host plant Pisum sativum L. In parallel experiments, virions of another potyvirus, potato virus Y (PVY), infected tobacco protoplasts but not protoplasts from pea, a nonhost for PVY. The results suggest, that the inabihty of PSbMV to infect tobacco, and of PVY to infect pea, is at least partly caused by a failure of virus replication in the inoculated cells.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Merete Albrechtsen

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Potato virus Y
	Pea seed-borne mosaic virus