Journal of Phytopathology (2004) 152, 575-579

B. Duduk, S. Botti, M. Ivanovic, B. Krstic, N. Dukic and A. Bertaccini (2004)
Identification of phytoplasmas associated with grapevine yellows in Serbia
Journal of Phytopathology 152 (10), 575-579
Abstract: The molecular identification and characterization of phytoplasmas from infected grapevines in four locations in Serbia are reported. Phytoplasmas were detected and identified by restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified 16S rDNA. Grapevine yellows were associated with three molecularly distinguishable phytoplasmas: Flavescence dorée phytoplasmas (elm yellows group: 16SrV-C subgroup) were present only in the Zupa Aleksandrovac region; Bois noir phytoplasmas (stolbur group: 16SrXII-A subgroup) were detected in the other surveyed regions; a mixed infection of European stone fruit yellows (apple proliferation group: 16SrX-B subgroup) and Bois noir phytoplasmas was identified in one sample. A finer molecular characterization by RFLP analysis of rpS3 and SecY genes of Flavescence dorée phytoplasmas from Zupa Aleksandrovac confirmed that the Serbian genotype is indistinguishable from a strain from the Veneto region, Italy. Characterization of the tuf gene of Bois noir phytoplasmas showed lack of amplification of samples from Erdevik. HpaII profiles of tuf gene PCR products of samples from Pali and Radmilovac were identical, and were indistinguishable from one of the two profiles produced by samples from Italian grapevines used as reference strains.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Bojan Duduk, Assunta Bertaccini

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
general biology - morphology - evolution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytoplasma vitis	Grapevine (Vitis)	Italy
	Phytoplasma vitis	Grapevine (Vitis)	Serbia
	Phytoplasma prunorum		Serbia
	Phytoplasma solani	Grapevine (Vitis)	Serbia