Journal of Pest Science (2012) 85, 227-238

G. Parrella, L. Scassillo and M. Giorgini (2012)
Evidence for a new genetic variant in the Bemisia tabaci species complex and the prevalence of the biotype Q in southern Italy
Journal of Pest Science 85 (2), 227-238
Abstract: The genetic diversity of Bemisia tabaci was investigated in Southern Italy using the mitochondrial cytochrome oxidase I (COI) gene as molecular marker and sampling whiteflies on cultivated plants, weeds and bushes. Phylogenetic analysis of COI sequences and restriction analysis of COI amplicons were used to genotype whitefly populations. A PCR-RFLP method based on digestion with the endonuclease ApoI was set up to identify the B. tabaci genetic variants so far recorded from the Mediterranean region. In general, biotype Q populations were most frequently collected (76.0% of all sampled populations). In greenhouse districts, 87.0% sampled populations were identified as biotype Q, and 13.0% were assigned to biotype B. Outside the greenhouse districts, the biotype B was never collected, whilst biotype Q populations were found on weeds and on plants cultivated in family gardens in different environments, also located in interior plains or in mountain areas distant from intensive cultivations of whitefly host plants. A new genetic variant unrelated to B and Q biotypes, which was named Ru, was collected on Rubus ulmifolius and grapevine. Phylogenetic analysis of COI sequences shown that Ru haplotypes form a well-supported clade sister to the clade including the Asian/Australian major genetic groups and the Italy major genetic group. The closest relative of the Ru clade (10.7% pairwise genetic distance) was the Italy group, with this latter so far including only the haplotypes of the T biotype. These results were discussed in the light of the recent B. tabaci species concept.
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Link to article at publishers website
Database assignments for author(s): Giuseppe Parrella, Massimo Giorgini

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bemisia tabaci biotype MEAM1		Italy
	Bemisia tabaci biotype MED		Italy