Journal of Medical Entomology (2002) 39, 248-250

Michael J. Turell, John C. Morrill, Cynthia A. Rossi, Adel M. Gad, Stanton E. Cope, Tamara L. Clements, Ray R. Arthur, Leonard P. Wasieloski, David J. Dohm, Denise Nash, Mosaad M. Hassan, Ali N. Hassan, Zakaria S. Morsy and Steven M. Presley (2002)
Isolation of West Nile and Sindbis viruses from mosquitoes collected in the Nile valley of Egypt during an outbreak of Rift Valley fever
Journal of Medical Entomology 39 (1), 248-250
Abstract: As part of an evaluation of potential vectors of arboviruses during a Rift Valley fever (RVF) outbreak in the Nile Valley of Egypt in August 1993, we collected mosquitoes in villages with known RVF viral activity. Mosquitoes were sorted to species, pooled, and processed for virus isolation both by intracerebral inoculation into suckling mice and by inoculation into cell culture. A total of 33 virus isolates was made from 36,024 mosquitoes. Viruses were initially identified by indirect fluorescent antibody testing and consisted of 30 flaviviruses (all members of the Japanese encephalitis complex, most probably West Nile [WN] virus) and three alphaviruses (all members of western equine encephalitis complex, most probably Sindbis). The identity of selected viruses was confirmed by reverse transcriptase-polymerase chain reaction and sequencing. Culex antennatus (Becker) and Culex perexiguus Theobald accounted for five (17%) and 23 (77%) of the WN virus isolations, respectively. Despite isolation of viruses from 32 pools of mosquitoes (both WN and Sindbis viruses were isolated from a single pool), RVF virus was not isolated from these mosquitoes, even though most of them are known competent vectors collected during an ongoing RVF outbreak. Thus, it should be remembered, that even during a known arbovirus outbreak, other arboviruses may still be circulating and causing disease.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Michael J. Turell, David J. Dohm

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Culex antennatus		Egypt
	Culex perexiguus		Egypt