Journal of Insect Science (2005) 5 (44), 1-8

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L.M. Seymour, T.M. Mowry, W.H. Day and J.D. Barbour (2005)
Parasitism of Lygus spp. nymphs by the parasitoid wasp, Peristenus howardi, in the alfalfa seed-growing region of the Pacific Northwest
Journal of Insect Science 5 (44), 1-8
Abstract: The parasitoid, Peristenus howardi Shaw (Hymenoptera: Braconidae) has been found to parasitize a large proportion of Lygus species in some Washington and Idaho alfalfa seed fields. During 2002-2003 a survey was conducted to estimate the proportion of Lygus spp. (Hemiptera: Miridae) parasitized and the amount of that parasitism attributable to P. howardi in crop and non-crop plants attacked by Lygus in the alfalfa seed growing region of southwestern Idaho and eastern Oregon. Percentage parasitism was estimated from dissection of field-collected Lygus nymphs. Polymerase chain reaction (PCR) was used to amplify DNA extracted from parasitoid larvae followed by restriction endonuclease digestion of PCR products to distinguish P. howardi from other potentially co-occurring Peristenus species. Peak parasitism of Lygus nymphs occurred between the first and third weeks of July for both years for all host plants sampled. Of the parasitoid larvae recovered from Lygus nymphs in our study, 75% to 80% tested positive as Peristenus spp. and 76% of these matched the endonuclease digestion banding pattern for P. howardi. The identity of the remaining 20% to 25% of the parasitoids is not known.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: English)
Link to article at publishers website


Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
surveys/distribution/isolation


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Lygus hesperus Alfalfa/lucerne (Medicago sativa) U.S.A. (NW)
Peristenus howardi (parasitoid) U.S.A. (NW)
Peristenus mellipes (parasitoid) U.S.A. (NW)