Journal of Fungi (2023) 9 (4 - 489)

Yu-Hang Qiao, Chen-Ning Zhang, Min Li, Huan Li, Yun-Fei Mao and Feng-Mao Chen (2023)
Species of the Colletotrichum spp., the causal agents of leaf spot on European hornbeam (Carpinus betulus)
Journal of Fungi 9 (4 - 489)
Abstract: European hornbeam (Carpinus betulus L.) is widely planted in landscaping. In October 2021 and August 2022, leaf spot was observed on C. betulus in Xuzhou, Jiangsu Province, China. To identify the causal agent of anthracnose disease on C. betulus, 23 isolates were obtained from the symptomatic leaves. Based on ITS sequences and colony morphology, these isolates were divided into four Colletotrichum groups. Koch's postulates of four Colletotrichum species showed similar symptoms observed in the field. Combining the morphological characteristics and multi-gene phylogenetic analysis of the concatenated sequences of the internal transcribed spacer (ITS) gene, Apn2-Mat1-2 intergenic spacer (ApMat) gene, the calmodulin (CAL) gene, glyceraldehyde3-phosphate dehydrogenase (GAPDH) gene, Glutamine synthetase (GS) gene, and beta-tubulin 2 (TUB2) genes, the four Colletotrichum groups were identified as C. gloeosporioides, C. fructicola, C. aenigma, and C. siamense. This study is the first report of four Colletotrichum species causing leaf spot on European hornbeam in China, and it provides clear pathogen information for the further evaluation of the disease control strategies.
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Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Colletotrichum gloeosporioides	Hornbeam (Carpinus)	China (NE)
	Colletotrichum fructicola	Hornbeam (Carpinus)	China (NE)
	Colletotrichum siamense	Hornbeam (Carpinus)	China (NE)
	Colletotrichum aenigma	Hornbeam (Carpinus)	China (NE)