Intervirology (1977) 8, 117-121

A.M. Crawford, J. Kalmakoff and J.F. Longworth (1977)
A solid-phase radioimmunoassay for polyhedron protein from baculoviruses
Intervirology 8 (2), 117-121
Abstract: A solid-phase radioimmunoassay has been developed for detecting polyhedron protein of Wiseana spp. nuclear polyhedrosis virus (W.NPV) using antigen fixed to plastic microtiter wells. The direct assay using ¹²⁵I-labeled rabbit immunoglobulins could detect 0.2 µg of polyhedron protein, and the indirect method using ¹²⁵I-labeled sheep anti-rabbit immunoglobulins could detect 0.05 µg of polyhedron protein. Antibodies against W.NPV polyhedron protein could be detected using a competition assay with labeled and unlabeled antibodies, providing a method suitable for rapid and quantitative screening of sera.
(The abstract has been supplied by the author or ISPI and is excluded from the Creative Commons licence.)
(original language: English)
Link to article at publishers website
Database assignments for author(s): Allan M. Crawford

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy
surveys/distribution/isolation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Wiseana (genus)	Grasses/turf/rangeland	New Zealand
Alphabaculovirus wisignatae (entomopathogen)	Wiseana (genus)	Grasses/turf/rangeland	New Zealand