International Journal of Pest Management (2013) 59, 329-338
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Molecular characterization of genetic diversity within the Africa/Middle East/Asia Minor and Sub-Saharan African groups of the Bemisia tabaci species complex
International Journal of Pest Management 59 (4), 329-338
Abstract: Bemisia tabaci Gennadius is a major plant pest in many agricultural systems worldwide. It is a complex of cryptic species that differ in many ecological respects, including damage-causing potential. Monitoring the genetic composition of B. tabaci populations in the field is, therefore, essential for achieving effective control. Sequencing is costly and time consuming; thus, efficient diagnostic tools must be developed to perform epidemiological studies involving hundreds of individuals. Here, we describe a polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method for identifying all the putative species comprising the Africa/Middle East/Asia Minor group, including those that are the most devastating crop pests. Moreover, intra-specific diversity associated with specific symbiotic bacteria known to manipulate insect host phenotypes can also be detected. In addition, this method discriminates between the Africa/Middle East/Asia Minor and the Sub-Saharan African groups, which are sympatric in some areas. This simple, reliable and cost-effective diagnostic tool is ideal for the rapid analysis of a high number of individuals and, thus, has potential applications in field contexts, where it could provide valuable baseline information for pest management programs.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Olivier Gnankine
Research topic(s) for pests/diseases/weeds:
identification/taxonomy
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant. |
|---|---|---|---|---|
| Bemisia tabaci biotype MEAM1 | ||||
| Bemisia tabaci biotype MED |
