International Journal of Nematology (2002) 12, 35-40

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H.S.A. Salama, M.M.M. Abd-Elgawad and Magda A. Mohamed (2002)
Characterization of Egyptian heterorhabditid nematodes through enzymes and soluble proteins
International Journal of Nematology 12 (1), 35-40
Abstract: Five Egyptian isolates of heterorhabditid nematodes were compared by SDS-PAGE for soluble proteins with previously identified Egyptian Heterorhabditis bacteriophora and H. indica. Three isolates were more closely related in the number of bands in the pattern than the rest though their common bands related them to H. bacteriophora. The same observation applied to another isolate but its common bands related it to H. indica. The fifth isolate showed to some extent dissimilarity with both the species. Quantitative determination of catalase, protease, lipase and esterase varied in six of the seven, nematode isolates. Differences were found among nematode isolates in their specific activity of caralase which may represent the biochemical mean required to overcome the host antioxidant enzymes for defence. The isolate H. bacteriophora AS 1 demonstrated high levels of both lipase and esterase. Total activity of lipase and esterase per 10,000 non-feeding infective stage juveniles ranged from 60:9±5.4 to 190±17.6 and 56.3±4.0 to 183.9±18 units; respectively indicating varying degrees of lipid hydrolysis. H. bacteriophora AS 1 demonstrated the highest proteolytic activity which may reflect the ability of nematodes to penetrate into the insect haemocoel by the release of proteases. A possible variation among the nematode isolates in their persistence and ability to infect their insect hosts in the light of differences in the levels of lipase, esterase, catalase and protease was discussed.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): H.S. Salama, Mahfouz M.M. Abd-Elgawad

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Heterorhabditis indica/Photorhabdus (entomopathogen) Egypt
Heterorhabditis bacteriophora/Photorhabdus (entomopathogen) Egypt
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