In Vitro - Plant (1982) 18, 813-816

Allan M. Crawford (1982)
A coleopteran cell line derived from Heteronychus arator (Coleoptera: Scarabaeidae)
In Vitro - Plant 18 (10), 813-816
Abstract: The first continuous coleopteran cell line, designated DSIR-HA-1179, was derived from the scarab beetle Heteronychus arator. Most cells are spindle-shaped, 25 to 40 µm long and 15 to 20 µm wide, adhere strongly to plastic surfaces, and are highly resistant to trypsin and collagenase treatment. The population doubling time of 6 d, at 27° C, is slow compared with most dipteran and lepidopteran cell lines. The cells cross-react in comparative radioimmunoassays with H. arator larval tissue but not other insect cell lines and support the replication of Oryctes baculovirus and at least two nodaviruses, black beetle virus, and Flock House virus.
(The abstract has been supplied by the author or ISPI and is excluded from the Creative Commons licence.)
(original language: English)
Link to article at publishers website
Database assignments for author(s): Allan M. Crawford

Research topic(s) for pests/diseases/weeds:
rearing/culturing/mass production
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
rearing/culturing/mass production

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Heteronychus arator
Black beetle virus (entomopathogen)
Alphanudivirus oryrhinocerotis (entomopathogen)