HortScience (2005) 40, 694-696

Charles A. Powell and Youjian Lin (2005)
Separation of Citrus tristeza virus isolates in mixed infections through transfer by single brown citrus aphids
HortScience 40 (3), 694-696
Abstract: One hundred single brown citrus aphid (BCA) (Toxoptera citricida Kirkaldy) transmission attempts were made from each of 16 different citrus trees [8 grapefruit (Citrus paradisi Macf.) and 8 sweet orange (C. sinensis (L.) Osbeck)] previously inoculated with decline-inducing (T36-CTV), non-decline-inducing (T30-CTV), a mixture of the two Citrus tristeza virus isolate types, or no CTV. Successful CTV transmission occurred in 1.5% of attempts from grapefruit trees that had been bark-chip-inoculated with T36-CTV, 3% of attempts from orange trees inoculated with T36-CTV, 3% of attempts from grapefruit trees inoculated with both T36- and T30-CTV, 4% of attempts from orange trees inoculated with both T36- and T30-CTV, 1.5% of attempts from grapefruit trees inoculated with T30-CTV, and 3.5% of attempts from orange trees inoculated with T30-CTV. Single BCA were able to recover T30-like-CTV from trees believed to be inoculated only with T36-CTV, and T36-like-CTV from trees believed to be inoculated only with T30-CTV, suggesting that these inoculum sources were also mixtures of T36-CTV and T30-CTV. The T36-CTV was not immunologically detectable in some of the trees from which it was transmitted indicating that single BrCA can recover T36-CTV from a T36-CTV/T30-CTV mixture in which the T36-CTV is an undetectable, minority component.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aphis citricidus	Citrus (genus)	U.S.A. (SE)
	Citrus tristeza virus	Citrus (genus)	U.S.A. (SE)