Formosan Entomologist (2005) 25, 243-254

Kivem Hsu, Tsen Hua, Niann-Tai Chang and Wen-Bin Yeh (2005)
[Molecular identification of important spider mites using multiplex PCR and PCR-RFLP]
Formosan Entomologist 25 (4), 243-254
Abstract: Species recognition of spider mites is difficult due to their small body sizes and unstable morphological characters in the immature stages. In this study, sequences of 28S rDNA and ITS2 of ~2900 and 600 bp in length, respectively, were used to identify the molecular variations of five species of spider mites in four genera: Oligonychus coffeae (Nietner), Panonychus citri, Panonychus ulmi (Koch), Petrobia harti (Ewing), and Tetranychus kanzawai Kishida. Specific primers and restricted digestion enzymes were designed from the 28S rDNA and ITS2 regions, and applied for multiplex PCR and PCR-RFLP to identify these spider mites. Results showed that the application of the two molecular markers was useful for identifying these economically important pests.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Wen-Bin Yeh, Niann-Tai Chang

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Panonychus ulmi		Taiwan
	Panonychus citri		Taiwan
	Tetranychus kanzawai		Taiwan
	Oligonychus coffeae		Taiwan
	Tetranychina harti		Taiwan