Florida Entomologist (2011) 94, 356-358

Nurmastini Sufina Bujang, Nigel A. Harrison and Nan-Yao Su (2011)
An improved method for extraction and purification of termite endo-β-1,4-glucanase from FTA® cards
Florida Entomologist 94 (2), 356-358
Abstract: In conclusion, we report an improved method for extraction and purification of termite nucleic acid from the FTA® Plantsaver Card (Whatman), which involves elution with TE buffer (pH 8) and purification with Wizard® DNA Clean-Up System (Promega). Our DNA recovery protocol requires less material, equipment, preparation, and manipulation, and reduces processing time, costs and chances for contamination compared with the manufacturer-recommended FTA® purification protocol. Most important, in relation to our study, it has been consistently effective in obtaining termite nucleic acid permitting further DNA analysis when all other methods have failed.
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Database assignments for author(s): Nan-Yao Su, Nigel A. Harrison

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Macrotermes gilvus		Malaysia
	Nasutitermes corniger
	Macrotermes carbonarius		Malaysia
	Microcerotermes crassus		Malaysia