Fitopatologia Brasileira (2002) 27, 631-634

Virgínia C. de Oliveira and Jefferson L.S. da Costa (2002)
Análise de restrição de DNA ribossomal amplificado (ARDRA) pode diferenciar Fusarium solani f. sp. phaseoli de F. solani f. sp. glycines
[Restriction analysis of rDNA (ARDRA) can differentiate Fusarium solani f. sp. phaseoli from F. solani f. sp. glycines]
Fitopatologia Brasileira 27 (6), 631-634
Abstract: Molecular methods have been used to characterize diversity among pathogenic and non pathogenic isolates of Fusarium spp. and to determine genetic relationships among formae speciales. Pathogenicity tests performed on dry beans (Phaseolus vulgaris) and soybeans (Glycine max) with 17 isolates of Fusarium solani did not demonstrate host specificity. Amplified Ribosomal DNA Restriction Analysis (ARDRA) was used to analyze the ITS1 - 5,8S rDNA - ITS2 region, amplified with primers ITS4 and ITS5. The amplified products were digested with the restriction enzymes Hae III and Msp I. Banding patterns generated by the enzyme Hae III enabled the differentiation of three groups within F. solani, one specific for isolates of F. solani f. sp. phaseoli and the other two for F. solani f. sp. glycines. The ARDRA technique, using the enzyme Hae III, is a promising marker to differentiate the formae speciale sphaseoli from glycines within the F. solani complex.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
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Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Soybean sudden death syndrome	Soybean (Glycine max)	Brazil (south)
	Soybean sudden death syndrome	Beans (Phaseolus)
	Neocosmospora phaseoli	Soybean (Glycine max)
	Neocosmospora phaseoli	Beans (Phaseolus)	Brazil (south)