Canadian Journal of Plant Pathology (2014) 36, 216-223

S.S. Egamberdiev, I.B. Salahutdinov, A.A. Abdullaev, M. Ulloa, S. Saha, F. Radjapov, B. Mullaohunov, D. Mansurov, J.N. Jenkins and I.Y. Abdurakhmonov (2014)
Detection of Fusarium oxysporum f. sp. vasinfectum race 3 by single-base extension method and allele-specific polymerase chain reaction
Canadian Journal of Plant Pathology 36 (2), 216-223
Abstract: Allele specific (AS) SNP primers were developed for rapid detection of Fusarium oxysporum f.sp. vasinfectum (FOV) race 3. These primers were designed based on single nucleotide polymorphisms of partial sequence alignment of the β-tubulin (BT) gene from several FOV races. The primers showed high specificity for FOV race 3, and exclusively amplified PCR products from FOV race 3 DNAs and total genomic DNAs of FOV race 3-infected plant tissues. These primers did not produce any amplification products for races 1, 2, 4, 6, 7 and 8 of FOV or other FOV and Fusarium species from infected plant tissues. The assay is useful for specific and rapid detection of Fusarium oxysporum f. sp. vasinfectum race 3.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Mauricio Ulloa

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium oxysporum
	Fusarium gossypinum