Canadian Journal of Botany - Revue Canadienne de Botanique (1994) 72, 370-377

Robert S. Jeng and Clive M. Brasier (1994)
Two-dimensional mapping of mycelial polypeptides of Ophiostoma ulmi and Ophiostoma novo-ulmi, causal agents of Dutch elm disease
Canadian Journal of Botany - Revue Canadienne de Botanique 72 (3), 370-377
Abstract: Two-dimensional mycelial polypeptide maps were used to compare interspecific and intraspecific relationships among six isolates of Ophiostoma ulmi and four Eurasian and four North American race isolates of Ophiostoma novo-ulmi. The basic polypeptide pattern of O. ulmi differs markedly from that of O. novo-ulmi. A number of polypeptides were identified that could differentiate the two species and one was detected that may discriminate Eurasian from North American races. Overall, a high degree of correlation between species, race, and polypeptides was evident. However, two O. ulmi isolates (MAB and M273) and one O. novo-ulmi isolate (83) exhibited the same unique polypeptides. These isolates otherwise exhibit typical O. ulmi or O. novo-ulmi nuclear DNA polymorphisms. This suggests that they may share a common developmental pathway absent in other isolates examined. In view of this phenomenon, the greatest value of two-dimensional polypeptide gels may lie in the elucidation of proteins involved in species or race specificity, in pathogenesis, or in development rather than in routine species identification.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Clive M. Brasier

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ophiostoma ulmi
	Ophiostoma novo-ulmi