Bulletin of Insectology (2011) 64, S41-S42

Jennifer Hodgetts, Jenny Tomlinson, Neil Boonham, Irene González-Martín, Petra Nikolić, Philip Swarbrick, Egya N. Yankey and Matthew Dickinson (2011)
Development of rapid in-field loop-mediated isothermal amplification (LAMP) assays for phytoplasmas
Bulletin of Insectology 64 (Suppl.), S41-S42
Second International Phytoplasmologist Working Group Meeting (IPWG), Neustadt an der Weinstraße (Germany) September 12-15, 2011
Abstract: Loop-mediated isothermal amplification (LAMP) is an isothermal amplification technique that can be undertaken with minimal equipment to obtain amplification of target DNA within 30 minutes. A range of assays for specific 16Sr phytoplasma groups, which when combined with rapid DNA extraction techniques can result in detection of the phytoplasma in plant material within 1 hour of sampling was developed. A range of alternative methods are available for detection of the amplification product including incorporation of hydroxyl napthol blue into the reagent mix, agarose gel electrophoresis and real-time detection systems. The advantage of the real-time method is that tubes do not have to be opened, minimising the risk of contamination of samples.
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Database assignments for author(s): Jennifer Hodgetts, Neil Boonham, Jenny A. Tomlinson

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	16SrI phytoplasma group
	16SrII phytoplasma group
	16SrXII phytoplasma group
	16SrV phytoplasma group
	16SrIII phytoplasma group
	16SrXI phytoplasma group
	16SrXXII phytoplasma group