Bulletin of Insectology (2011) 64, S35-S36

Mogens Nicolaisen, Nicoletta Contaldo, Olga Makarova, Samanta Paltrinieri and Assunta Bertaccini (2011)
Deep amplicon sequencing reveals mixed phytoplasma infection within single grapevine plants
Bulletin of Insectology 64 (Suppl.), S35-S36
Second International Phytoplasmologist Working Group Meeting (IPWG), Neustadt an der Weinstraße (Germany) September 12-15, 2011
Abstract: The diversity of phytoplasmas within single plants has not yet been fully investigated. In this project, deep amplicon sequencing was used to generate 50,926 phytoplasma sequences from 11 phytoplasma-infected grapevine samples from a PCR amplicon in the 5' end of the 16S region. After clustering and alignment to phytoplasma reference sequences it was shown that the phytoplasmas in the 11 plants belonged to diverse 16Sr groups and that a high number of single nucleotide polymorphisms were present.
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Database assignments for author(s): Olga Makarova, Assunta Bertaccini, Nicoletta Contaldo, Mogens Nicolaisen

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytoplasma prunorum	Grapevine (Vitis)	Italy
	16SrXII phytoplasma group	Grapevine (Vitis)	Italy
	16SrV phytoplasma group	Grapevine (Vitis)	Italy