BioControl (2011) 56, 265-275

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Paul F. Rugman-Jones, Lisa D. Forster, Emilio Guerrieri, Robert F. Luck, Joseph G. Morse, Maurilia M. Monti and Richard Stouthamer (2011)
Taxon-specific multiplex-PCR for quick, easy, and accurate identification of encyrtid and aphelinid parasitoid species attacking soft scale insects in California citrus groves
BioControl 56 (3), 265-275
Abstract: Citricola scale, Coccus pseudomagnoliarum Kuwana (Hemiptera: Coccidae), is a serious pest of citrus in California's San Joaquin Valley, but not in southern California where a complex of Metaphycus spp. Mercet (Hymenoptera: Encyrtidae) suppress it. This has created interest in using these (and other Metaphycus) species for biological control in the San Joaquin Valley. A critical step in assessing an organism's potential for biological control is the ability to accurately identify it. For Metaphycus spp., this currently requires slide mounted adult specimens and expert taxonomic knowledge. We present a simple, quick and accurate method to identify any life stage of the ten major parasitoids of soft scales in California citrus, based on amplification of ribosomal DNA, using the polymerase chain reaction (PCR). Three multiplex-PCR protocols amplify products of taxon-specific sizes, allowing direct diagnosis of taxa accommodated by the PCR, and reducing identification time to a fraction of that of existing methods.
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Full text of article
Database assignments for author(s): Paul F. Rugman-Jones, Richard Stouthamer, Emilio Guerrieri

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy
surveys/distribution/isolation


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Coccus pseudomagnoliarum Citrus (genus) U.S.A. (SW)
Metaphycus helvolus (parasitoid) Coccus pseudomagnoliarum Citrus (genus) U.S.A. (SW)
Coccophagus lycimnia (parasitoid) U.S.A. (SW)
Microterys nietneri (parasitoid) U.S.A. (SW)
Metaphycus flavus (parasitoid) U.S.A. (SW)
Metaphycus luteolus (parasitoid) Coccus pseudomagnoliarum Citrus (genus) U.S.A. (SW)
Metaphycus stanleyi (parasitoid) U.S.A. (SW)
Coccophagus semicircularis (parasitoid) U.S.A. (SW)
Coccophagus cowperi (parasitoid) U.S.A. (SW)
Metaphycus angustifrons (parasitoid) U.S.A. (SW)
Coccophagus rusti (parasitoid) U.S.A. (SW)