BioControl (2010) 55, 423-434

John Mukuka, Olaf Strauch, Lieven Waeyenberge, Nicole Viaene, Maurice Moens and Ralf-Udo Ehlers (2010)
Heat tolerance among different strains of the entomopathogenic nematode Heterorhabditis bacteriophora
BioControl 55 (3), 423-434
Abstract: Quality of biological control products based on entomopathogenic nematodes can be severely damaged due to exposure to high temperature surpassing 40°C. The study screened 36 natural populations and 18 hybrid or inbred strains of Heterorhabditis bacteriophora for their response to high temperature. Nematodes were tested with or without prior adaptation to heat at 35°C for 3 h. Five strains of H. indica and one of H. megidis were also included. Molecular identification using nuclear ribosomal DNA sequences confirmed the designation to the three Heterorhabditis spp. The mean tolerated temperature ranged from 33.3°C to 40.1°C for non-adapted and from 34.8°C to 39.2°C for adapted strain populations. H. indica was the most tolerant, followed by H. bacteriophora and H. megidis. No correlation was recorded between tolerance assessed with and without adaptation to heat, implying that different genes are involved. Correlation between heat tolerance and mean annual temperature at place of origin of the strains was weak. A high variability in tolerance among strains and the relatively high heritability (h² = 0.68) for the adapted heat tolerance recorded for H. bacteriophora provide an excellent foundation for future selective breeding with the objective to enhance heat tolerance of H. bacteriophora.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Ralf-Udo Ehlers, Lieven Waeyenberge, Nicole M. Viaene

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
environment/habitat manipulation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Heterorhabditis indica/Photorhabdus (entomopathogen)
Heterorhabditis bacteriophora/Photorhabdus (entomopathogen)