BMC Malaria Journal (2017) 16 (351) - 2-Butanone as a carbon dioxide ...

Monicah M. Mburu, Collins K. Mweresa, Philemon Omusula, Alexandra Hiscox, Willem Takken and Wolfgang R. Mukabana (2017)
2-Butanone as a carbon dioxide mimic in attractant blends for the Afrotropical malaria mosquitoes Anopheles gambiae and Anopheles funestus
BMC Malaria Journal 16 (351)
Abstract:
Background
Most odour baits designed to attract host-seeking mosquitoes contain carbon dioxide (CO₂), which enhances trap catches, given its role as a mosquito flight activator. However, the use of CO₂ is expensive and logistically demanding for prolonged area-wide use.
Methods
This study explored the possibility of replacing organically-produced CO₂ with 2-butanone in odour blends targeting host-seeking malaria mosquitoes. During semi-field and field experiments MM-X traps were baited with a human odour mimic (MB5 blend) plus CO₂ or 2-butanone at varying concentrations. Unbaited traps formed a control. The attraction of Anopheles gambiae s.s., Anopheles arabiensis and Anopheles funestus to these differently baited traps was measured and mean catch sizes were compared to determine whether 2-butanone could form a viable replacement for CO₂ for these target species.
Results
Under semi-field conditions significantly more female An. gambiae mosquitoes were attracted to a reference attractant blend (MB5 + CO₂) compared to MB5 without CO₂ (P < 0.001), CO₂ alone (P < 0.001), or a trap without a bait (P < 0.001). Whereas MB5 + CO₂ attracted significantly more mosquitoes than its variants containing MB5 plus different dilutions of 2-butanone (P = 0.001), the pure form (99.5%) and the 1.0% dilution of 2-butanone gave promising results. In the field mean indoor catches of wild female An. gambiae s.l. in traps containing MB5 + CO₂ (5.07 ± 1.01) and MB5 + 99.5% 2-butanone (3.10 ± 0.65) did not differ significantly (P = 0.09). The mean indoor catches of wild female An. funestus attracted to traps containing MB5 + CO₂ (3.87 ± 0.79) and MB5 + 99.5% 2-butanone (3.37 ± 0.70) were also similar (P = 0.635). Likewise, the mean outdoor catches of An. gambiae and An. funestus associated with MB5 + CO₂ (1.63 ± 0.38 and 0.53 ± 0.17, respectively) and MB5 + 99.5% 2-butanone (1.33 ± 0.32 and 0.40 ± 0.14, respectively) were not significantly different (P = 0.544 and P = 0.533, respectively).
Conclusion
These results demonstrate that 2-butanone can serve as a good replacement for CO₂ in synthetic blends of attractants designed to attract host-seeking An. gambiae s.l. and An. funestus mosquitoes. This development underscores the possibility of using odour-baited traps (OBTs) for monitoring and surveillance as well as control of malaria vectors and potentially other mosquito species.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Wolfgang Richard Mukabana, Willem Takken

Research topic(s) for pests/diseases/weeds:
pheromones/attractants/traps

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles gambiae		Kenya
	Anopheles funestus		Kenya