Applied and Environmental Microbiology (2011) 77, 2667-2675

Imma Güell, Jordi Cabrefiga, Esther Badosa, Rafael Ferre, Montserrat Talleda, Eduard Bardají, Marta Planas, Lidia Feliu and Emilio Montesinos (2011)
Improvement of the efficacy of linear undecapeptides against plant-pathogenic bacteria by incorporation of D-amino acids
Applied and Environmental Microbiology 77 (8), 2667-2675
Abstract: A set of 31 undecapeptides, incorporating 1 to 11 D-amino acids and derived from the antimicrobial peptide BP100 (KKLFKKILKYL-NH₂), was designed and synthesized. This set was evaluated for inhibition of growth of the plant-pathogenic bacteria Erwinia amylovora, Pseudomonas syringae pv. syringae, and Xanthomonas axonopodis pv. vesicatoria, hemolysis, and protease degradation. Two derivatives were as active as BP100, and 10 peptides displayed improved activity, with the all-D isomer being the most active. Twenty-six peptides were less hemolytic than BP100, and all peptides were more stable against protease degradation. Plant extracts inhibited the activity of BP100 as well as that of the D-isomers. Ten derivatives incorporating one D-amino acid each were tested in an infectivity inhibition assay with the three plant-pathogenic bacteria by using detached pear and pepper leaves and pear fruits. All 10 peptides studied were active against E. amylovora, 6 displayed activity against P. syringae pv. syringae, and 2 displayed activity against X. axonopodis pv. vesicatoria. Peptides BP143 (KKLFKKILKYL-NH₂) and BP145 (KKLFKKILKYL-NH₂), containing one D-amino acid at positions 4 and 2 (underlined), respectively, were evaluated in whole-plant assays for the control of bacterial blight of pepper and pear and fire blight of pear. Peptide BP143 was as effective as streptomycin in the three pathosystems, was more effective than BP100 against bacterial blight of pepper and pear, and equally effective against fire blight of pear.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Emilio Montesinos, Jordi Cabrefiga

Research topic(s) for pests/diseases/weeds:
control - general

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Erwinia amylovora
	Pseudomonas syringae pv. syringae
	Xanthomonas vesicatoria
	Pseudomonas syringae