Applied and Environmental Microbiology (1999) 65, 3401-3406

E.T. Steenkamp, B.D. Wingfield, T.A. Coutinho, M.J. Wingfield and W.F.O. Marasas (1999)
Differentiation of Fusarium subglutinans f. sp. pini by histone gene sequence data
Applied and Environmental Microbiology 65 (8), 3401-3406
Abstract: Fusarium subglutinans f. sp. pini (= F. circinatum) is a pathogen of pine and is one of eight mating populations (i.e., biological species) in the Gibberella fujikuroi species complex. This species complex includes F. thapsinum, F. moniliforme (= F. verticillioides), F. nygamai, and F. proliferatum, as well as F. subglutinans associated with sugarcane, maize, mango, and pineapple. Differentiating these forms of F. subglutinans usually requires pathogenicity tests, which are often time-consuming and inconclusive. Our objective was to develop a technique to differentiate isolates of F. subglutinans f. sp. pini from other isolates identified as F. subglutinans. We sequenced the histone H3 gene from a representative set of Fusarium isolates. The H3 gene sequence was conserved and contained two introns in all the isolates studied. From both the intron and the exon sequence data, we developed a PCR-restriction fragment length polymorphism technique that reliably distinguishes F. subglutinans f. sp. pini from the other biological species in the G. fujikuroi species complex.
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Database assignments for author(s): Brenda D. Wingfield, Michael J. Wingfield, Teresa A. Coutinho

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium subglutinans	Maize/corn (Zea mays)	South Africa
	Fusarium subglutinans	Pineapple (Ananas comosus)
	Fusarium subglutinans	Mango (Mangifera indica)	South Africa
	Fusarium proliferatum	Rice (Oryza)	Taiwan
	Fusarium circinatum	Pine (Pinus)	South Africa
	Fusarium thapsinum
	Fusarium nygamai