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Applied Entomology and Zoology (2016) 51, 167-172

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A. Kurata, A. Fujiwara, N. Haruyama and T. Tsuchida (2016)
Multiplex PCR method for rapid identification of genetic group and symbiont infection status in Bemisia tabaci (Hemiptera: Aleyrodidae)
Applied Entomology and Zoology 51 (1), 167-172
Abstract: A serious pest insect Bemisia tabaci (Gennadius) consists of at least 24 genetic groups. In addition to the genetic groups, their symbiotic bacteria reportedly affect the ecological natures of B. tabaci such as the ability of these insects to transmit phytopathogenic viruses and resist insecticides. Thus, it is important to identify the genetic groups and the symbiont infection status for effective pest control. Here, we describe a multiplex diagnostic method that clearly identified three invasive and an indigenous genetic group (MEAM1, MED Q1, MED Q2 and Asia II 6) and seven symbionts (Portiera, Hamiltonella, Rickettsia, Cardinium, Wolbachia, Arsenophonus and Hemipteriphilus) of B. tabaci. Our methods enabled up to 83 % time saving and 67 % cost reduction, compared to the conventional methods, due to the following features: (1) crude DNA samples were used; (2) multiplex PCR greatly reduced the number of PCR experiments necessary; (3) restriction enzyme digestion was not required; (4) 250 V electrophoresis using sodium borate buffer yielded well-separated band patterns in only 10 min.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website


Research topic(s) for pests/diseases/weeds:
identification/taxonomy
general biology - morphology - evolution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Bemisia tabaci biotype MEAM1 Japan
Bemisia tabaci biotype MED Japan
Bemisia tabaci biotype China 1 China (south)
Bemisia tabaci biotype Asia II 6 Japan