Anais da Sociedade Entomológica do Brasil (1997) 26, 507-515

Andréia O. Gerk, Elliot W. Kitajima and Marlinda L. Souza (1997)
Identificação e Caracterização de Isolado Brasileiro do Vírus de Poliedrose Nuclear da Lagarta do Cartucho-do-Milho
[Identification and characterization of a Brazilian isolate of fall armyworm nuclear polyhedrosis virus]
Anais da Sociedade Entomológica do Brasil 26 (3), 507-515
Abstract: A nuclear polyhedrosis virus (NPV) from Spodoptera frugiperda (Smith) caterpillars (Lepidoptera: Noctuidae), collected from diseased insects in Sete Lagoas, MG, was confirmed and identified. The pathology of infected caterpillars is characteristic of NPV The virus showed tropism by ectoderm cells, fat cells, and tracheocytes. Viral particles (polyhedra and alkali released virions - ARVs) were purified through diferential centrifugations in sucrose gradient, and the polyhedral band was located in the inferior third part of the tube. Increasing time of polyhedral digestion with alkaline solution resulted in higher dissolution of occluded bodies (polyhedra) consequently releasing virions. Five bands were formed for the alkali released virions. The electrophoretic pattern of S. frugiperda NPV was compared to the one of Anticarsia gemmatalis (Hübner) NPV for polyhedra as well as ARVs. A peptide of 32,000 dalton was characterized as the polyhedrin of S. frugiperda NPV.
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(original language: Portuguese)
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Database assignments for author(s): Elliot W. Kitajima

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Spodoptera frugiperda		Brazil (south)
Alphabaculovirus spofrugiperdae (entomopathogen)	Spodoptera frugiperda		Brazil (south)