Anais da Sociedade Entomológica do Brasil (1997) 26, 121-132

Flávio Moscardi, Luiz G. Leite and Carlos E. Zamataro (1997)
Production of nuclear polyhedrosis virus of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae): Effect of virus dosage, host density and age
Anais da Sociedade Entomológica do Brasil 26 (1), 121-132
Abstract: The laboratory production of Anticarsia gemmatalis Hübner nuclear polyhedrosis virus (AgNPV) was evaluated for larvae inoculated at 1.0, 1.5, 2.0, and 2.5 cm and with two NPV dosages, 10⁶ and 10⁷ polyhedron inclusion bodies (PIB)/ml, sprayed on the insect diet surface. A fixed population of 25 larvae/300-ml cup was used. Highest virus yield was achieved for larvae inoculated with ca. 2.0 cm at the higher NPV dosage. Losses of NPV-inoculated larvae ranged from 42.4% to 64%, due to high incidence of canibalism; death by other causes, and pupation. In another experiment, larvae were inoculated with two NPV dosages (1.0 x 10⁷ and 4.0 x 10⁷ PIB/ml), using 25, 20, and 15 larvae/cup at the beginning of 4th instar (B4L); end of 4th instar (E4L); beginning of 5th instar (B5L); and end of 5th instar (E5L). Highest NPV production was attained for B5L at 25 larvae/cup, for both dosages, with a production efficiency of ca 90%, in spite of a decrease in PIB/larva and an increase in canibalism with the increase in larval density. Better standardization of larvae at inoculation unproved efficiency in NPV production.
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Database assignments for author(s): Luís G. Leite, Flavio Moscardi

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
rearing/culturing/mass production

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anticarsia gemmatalis
Alphabaculovirus angemmatalis (entomopathogen)	Anticarsia gemmatalis