Acta Entomologica Sinica (2006) 49, 754-758

Hong-Xiu Gao, Lan-Lan Han, Kui-Jun Zhao, Dong Fan and Jian Liu (2006)
[Cloning and sequencing of cytochrome oxidase II gene of Aphis glycines and its application in detecting natural enemies]
Acta Entomologica Sinica 49 (5), 754-758
Abstract: Cytochrome oxidase subunit II gene segment of Aphis glycines was cloned and sequenced. The length of the sequence was 810 bp (GenBank accession no. DQ265743). The sequence was contrasted with other aphids registered at GenBank to identify their molecular homology. One pair of sequence-characterized primer was developed, and its amplified single band was about 270 bp. The specificity tests performed with this pair of primer showed the presence of the band only in A. glycines. Molecular identification of the natural enemies fed on A. glycines was tested with the specific primer. The results indicated that the target DNA fragment of A. glycines was detected in guts of the predatory insects Harmonia axyridis, Propylaea japonica, Chrysopa septempunctata, and Orius similes, but not detected in the above predatory insects without feeding A. glycines.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aphis glycines
Harmonia axyridis (predator)	Aphis glycines
Propylea japonica (predator)	Aphis glycines
Orius strigicollis (predator)	Aphis glycines
Chrysopa pallens (predator)	Aphis glycines