Acta Entomologica Sinica (2000) 43, 127-133

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Guang-Mei Zhang, Shu-Sheng Liu and Raymond Akhurst (2000)
Analysis of five Chinese Bacillus thuringiensis formulations for the endotoxin components
Acta Entomologica Sinica 43 (2), 127-133
Abstract: In this paper, we investigated five Bacillus thuringiensis (Bt) strains from four mayor manufacturers of Bt products in China for the endotoxin components by PCR and SDS-PAGE. All five strains harbour cry1Aa and/or c, and/or d, and/or e genes, only the Bt + Virus strain has cry1Ab gene. The cry1A class of genes encodes for insecticidal crystal proteins with MW of 130 kD and toxic to Lepidoptera. The JS-Bt C strain contains the cry1B gene, which encodes for a 138 kD crystal protein, potentially toxic to both Lepidoptera and Coleoptera. Four of the five strains (HB-Bt P, Bt + Virus, JS-Bt P, and JS-Bt C) harbour the cry2Aa and/or b genes, which encode 70 kD crystal proteins toxic to Lepidoptera. All of the five strains have the cry1I gene which appears to be cryptic in all the strains. None of the five strains harbours the cry1C and cry1D genes.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Shu-Sheng Liu

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Bacillus thuringiensis Cry1A-toxin (entomopathogen)
Bacillus thuringiensis Cry1B-toxin (entomopathogen)
Bacillus thuringiensis Cry2A-toxin (entomopathogen)
Bacillus thuringiensis Cry1I-toxin (entomopathogen)