Journal of Pest Science (2009) 82, 47-53

From Pestinfo-Wiki
Revision as of 07:14, 3 July 2011 by Bernhard Zelazny (Talk | contribs) (command-line import)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Julia Janke, Martina Bandte, Christian Ulrichs, Giselher Grabenweger, Barbara Jäckel, Hartmut Balder and Carmen Büttner (2009)
Serological marking of Pnigalio agraules (Hymenoptera: Eulophidae) for field dispersal studies
Journal of Pest Science 82 (1), 47-53
Abstract: The parasitoid wasp Pnigalio agraules (Wlk.) is a key natural enemy of the horsechestnut leafminer Cameraria ohridella Deschka and Dimi (Lepidoptera: Gracillariidae). As a basis for mark-release-recapture studies, aimed at investigating the dispersal of this parasitoid in the field, adults of P. agraules were marked using a vertebrate-specific immunoglobulin (IgG). The marker was later detected by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). The IgG was either applied externally by spraying or internally by feeding an IgG-enriched diet. Different concentrations of the marker were used and the influence of abiotic (climatic conditions, time elapsed between marking and marker examination) and biotic factors (sex and age of the parasitoids) on the detection of the immunomarker was tested. External marking by spraying led to more homogeneous labelling than feeding the marker. Parasitoids labelled with 0.25 mg rabbit IgG per ten individuals contained enough immunomarker to be easily distinguished from unmarked ones. Neither the climatic conditions nor the sex or age of the insects had an influence on the detection of the marker. The IgG remained well detectable during the entire lifespan of the parasitoids, which was not negatively affected by the marking procedure. Serological marking can be used to investigate the dispersal behaviour of beneficial insects within mark-release-recapture studies.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Christian Ulrichs

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
population dynamics/epizootiology
inundative/inoculative release

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Cameraria ohridella
Pnigalio agraules (parasitoid) Cameraria ohridella
Retrieved from "https://wiki.pestinfo.org/w/index.php?title=Journal_of_Pest_Science_(2009)_82,_47-53&oldid=62296"