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Journal of Economic Entomology (1995) 88, 213-218

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Matthew H. Greenstone (1995)
Bollworm or budworm? Squashblot immunoassay distinguishes eggs of Helicoverpa zea and Heliothis virescens (Lepidoptera: Noctuidae)
Journal of Economic Entomology 88 (2), 213-218
Abstract: Management of pyrethroid resistance in the tobacco budworm, Heliothis virescens (F), requires some means to distinguish it from the cotton bollworm, Helicoverpa zea Boddie, referably at the egg stage. The subtle morphological differences that have been describe are not useful for field identification. Eggs squashed on nitrocellulose membranes can be unambiguously distinguished by a rapid assay that uses a monoclonal antibody to H. zea egg homogenate. Use of the assay to :identify eggs in the field would provide the data needed to make a decision on whether or not to spray pyrethroids. Adoption. of a resistance management strategy employing the assay in this fashion would delay the development of pyrethroid resistance in H. virescens, reduce wasteful spraying of pyrethroids and concomitant environmental pollution, save money otherwise lost by spraying the wrong insecticide, and enhance biological control by conserving natural enemies.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Matthew H. Greenstone

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest
surveys/sampling/distribution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Helicoverpa zea
Heliothis virescens