Journal of Applied Genetics (2011) 52, 233-243
Lidia Blaszczyk, Delfina Popiel, Jerzy Chelkowski, Grzegorz Koczyk, Gary J. Samuels, Krzysztof Sobieralski and Marek Siwulski (2011)
Species diversity of Trichoderma in Poland
Journal of Applied Genetics 52 (2), 233-243
Abstract: In the present study, we reinvestigate the diversity of Trichoderma in Poland utilizing a combination of morphological and molecular/phylogenetic methods. A total of 170 isolates were collected from six different substrata at 49 sites in Poland. These were divided among 14 taxa as follows: 110 of 170 Trichoderma isolates were identified to the species level by the analysis of their ITS1, ITS2 rDNA sequences as: T. harzianum (43 isolates), T. aggressivum (35), T. citrinoviride (11), T. hamatum (9), T. virens (6), T. longibrachiatum (4), T. polysporum (1), and T. tomentosum (1); 60 isolates belonging to the Viride clade were identified based on a fragment of the translation-elongation factor 1-alpha (tef1) gene as: T. atroviride (20 isolates), T. gamsii (2), T. koningii (17), T. viridescens (13), T. viride (7), and T. koningiopsis (1). Identifications were made using the BLAST interface in TrichOKEY and TrichoBLAST (http://www.isth.info). The most diverse substrata were soil (nine species per 22 isolates) and decaying wood (nine species per 75 isolates). The most abundant species (25%) isolated from all substrata was T. harzianum.
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Database assignments for author(s): Lidia Blaszczyk
Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
Research topic(s) for beneficials or antagonists:
surveys/distribution/isolation
