Journal of Applied Entomology (2009) 133, 602-614

H. Yu and F.-H. Wan (2009)
Cloning and expression of heat shock protein genes in two whitefly species in response to thermal stress
Journal of Applied Entomology 133 (8), 602-614
Abstract: Two whitefly species, Trialeurodes vaporariorum and Bemisia tabaci biotype B were shown to have different temperature tolerance and seasonal dynamics. To determine whether this variation in thermal tolerance is related to different expression patterns of heat shock protein (hsp) genes during temperature stress, we obtained complete cDNA sequences for hsp90, hsp70 and hsp20, and analysed their expression profiles across temperature gradients by real-time quantitative polymerase chain reaction (PCR). Six full-length cDNAs were cloned and sequenced from these two species. The full-length cDNAs of hsp90s contain 2166 and 2157 bp open-reading frames (ORF) which encode proteins with calculated molecular weights of 83 013 and 82 857 Da in T. vaporariorum and B. tabaci, respectively. The 1947 and 1959 bp ORFs of whitefly hsp70s comprise 649 and 653 amino acids with the calculated masses of 70 885 and 71 008 Da in T. vaporariorum and B. tabaci, respectively. Both complete cDNAs of hsp20 of T. vaporariorum and B. tabaci contain 585 bp ORFs and deduced amino acid sequences had molecular weights of 21 559 and 21 539 Da, respectively. The hsp expression profile results showed that temperatures for onset (Ton) or maximal (Tmax) induction of hsp expression in T. vaporariorum were generally 2-6°C lower than those in B. tabaci. These results suggest that the Ton (or Tmax) of hsps can represent the differences in temperature tolerance of these two whitefly species, and may be used to determine their natural geographical distribution and natural population seasonal dynamics. Significant upregulation of most hsps were observed when temperature stress was lifted, except that hsp70 and hsp20 of B. tabaci did not respond to the cold stress, indicating that response to heat and cold stress may have a different genetic and physiological basis in two whitefly species. These results highlight the importance of understanding the complexity of the heat shock response across multiple isoforms while attempting to link them to whole-organism traits such as thermal tolerance.
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Link to article at publishers website
Database assignments for author(s): Fang Hao Wan

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bemisia tabaci biotype MEAM1
	Trialeurodes vaporariorum